Rylan Schaeffer

Logo About Me
Resume
Research
Learning
Blog
Talks
Teaching
Jokes
Kernel Papers


29 March 2021

Creating a False Memory in the Hippocampus

by Ramirez and Liu, ..., Tonegawa (Science 2013)

Background

Previously, ablating/inactivating memory engram neurons prevented retrieval of fear response

Previous work by the Tonegawa lab showed that optogenetically activating memory engram cells (specifically in dentate gyrus (DG) during contextual fear conditioning) would reactive the fear memory.

Research Questions

Experiments

Exp 1 (Cellular)

Exp 2 (Behavioral)

The mouse is placed on DOX, then DOX is removed and the mouse is allowed to explore neutral context A. Later, in Context B, mouse is shocked while neurons tagged in context A are optogenetically activated. The mouse is then placed back in Context A and subsequently Context C.

Exp 3 (Behavioral)

Exp 4 (Behavioral)

Exp 5 (Neural)

Exp 6 (Neural, Behavioral)

Exp 7 (Neural, Behavioral)

tags: memory-engrams - basolateral-amygdala - central-amygdala - contextual-fear-conditioning